Hepes 250g (PH.EUR., USP) Oxygenate 500 ml Base A

Oxygenate 500 ml Base A for 10 min

autoclaved

Sulfites ppm                                          <= 15

Water %                                                7

Hepes 250g (PH.EUR., USP) Oxygenate 500 ml Base AHEPES (4 (2 Hydroxyethyl)piperazine 1 ethane sulfonic acid) has been described as one of the best all purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6. 0 to 8. 5. May be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen antibody reactions. HEPES has no nutritional benefit to cells. The